| Product Name | Methylene Blue Trihydrate |
| CAS Number | 7220-79-3 |
| Molecular Formula | C₁₆H₂₀ClN₃OS |
| Molecular Weight | 373.90 g/mol |
| Appearance | Dark green to blue-black crystalline powder |
| Assay (HPLC) | ≥ 98.0% |
| Loss on Drying | Corresponds to trihydrate content |
| pH (1% solution) | 3.0–4.5 |
| Heavy Metals | ≤ 10 ppm |
| Storage | Cool, dry, sealed, protected from light |
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Methylthioninium Chloride is a dark green to dark brown solid powder. Its aqueous solution is sky blue.
Its core characteristic is the dual nature of REDOX coloration – it remains stable blue in an oxidizing environment and turns colorless under reducing conditions.
The medical field focuses on specifically marking nerve and lymphoid tissues during the operation to enhance the accuracy of the operation.
The blue staining phenomenon of body fluids is used to assist in identifying ureteral fistula or bladder integrity, providing visual support for medical assessment.
At the same time, it can regulate the function of peripheral nerve ion channels to alleviate perceptual abnormalities and play a regulatory role in the microbial environment. It should be stored away from light.
Function:Detoxification and anti-inflammation

C16H20ClN3OS
Methylene Blue is a reversible redox indicator that cycles between oxidized (blue) and reduced (colorless/leuco) forms. This unique property, combined with its ability to cross biological membranes, makes it valuable for mitochondrial research, neuroscience, and antimicrobial studies.
Synonyms:
Basic Blue 9
Tetramethylthionine chloride
3,7-Bis(dimethylamino)phenazathionium chloride
Methylthioninium chloride
C.I. 52015 trihydrate
(7-Dimethylaminophenothiazin-3-ylidene)-dimethylazanium chloride trihydrate
Methylene Blue CP (Chemically Pure)
Available Forms:
| Form | CAS No. | Water Content | Best For |
|---|---|---|---|
| Methylene Blue Trihydrate | 7220-79-3 | 3 H₂O molecules | General research, staining, redox studies |
| Methylene Blue Anhydrous | 61-73-4 | 0 H₂O | Specialized applications |
| Methylene Blue Hydrate | 7220-79-3 (same) | Variable | See trihydrate |
LyvBio Co., Ltd. is a GMP-certified supplier of high-purity Methylene Blue Trihydrate for research and development applications.
Supply capabilities:
Purity: ≥98.0% (HPLC)
Form: Crystalline trihydrate
Monthly capacity: 100+ kg
Bulk quantities: 10g – 100kg
Lead time: 3–5 days (samples), 10–15 days (bulk)
Documentation: COA, HPLC chromatogram, stability data
Packaging: 50g, 100g, 500g, 1kg, 5kg, 10kg, 25kg (foil bag or HDPE drum)